Skip to main content
. 2018 Oct 23;33(5):429–439. doi: 10.1007/s12250-018-0054-x

Fig. 1.

Fig. 1

Preparation of rabbit polyclonal antibodies to PRRSV nsp8. (A) Analysis of the expression and purification of recombinant GST-nsp8 protein by 12% SDS-PAGE gel. (B) Western blot of the expression of GST-nsp8 by anti-GST mAb. (C) Immunofluorescence detection of HA-nsp8 in transfected MARC-145 cells by anti-GST-nsp8 serum. MARC-145 cells on coverslips in six well plates were transfected with the plasmid pHA-nsp8 (middle) or the vector pCMV-HA (right). At 18–24 h post transfection, the cells were fixed, permeablized and stained with proper antibodies to the HA epitope or to GST-nsp8, followed by Alexa Fluor 488-conjugated secondary antibodies. The cell nuclei were stained with Hoechst (blue) and examined by confocal microscopy. (D) Detection of nsp8 expression in PRRSV infected MARC-145 cells. MARC-145 cells grown on coverslips were either mock infected with DMEM or infected with HP-PRRSV strain JXwn06 at an MOI of 0.1. At 24 h after infection, the cells were fixed and stained with rabbit pre-immune serum or anti-GST-nsp8 serum and examined by confocal microscopy.