Fig. 6.

Role of MEK/ERK in the upregulation of p21 expression in AsPC-1 cells. Cells were stimulated by EGF (10 nM) or HGF (1 nM) for 24 h with or without SB203580 (10 μM) and/or PD98059 (20 μM) pretreatment for 30 min. (a) Immunoblots showing p21 expression and ERK phosphorylation. (b) Densitometric quantification of p21 expression based on three independent experiments. p21 expression was normalized to the level of GAPDH. The data represent the mean ± SEM of three independent experiments. * indicates p ≤ 0.05