Table 2.
Primer information for detecting SNPs in the coding sequences of Mx1 gene
| Primer sequences (5′-3′) | Annealing Temperature (℃) | Product(bp) | |
|---|---|---|---|
| Exon1 | F: CAGGAGGTAGAAGCATTGT | 61.2 | 431 |
| R: GGTGAGCATGGAGAGTATC | |||
| Exon2 | F: AGCATGAAGGTGCAAGGAT | 55.4 | 398 |
| R: TGAGTTTGTACCTTTGTCCCT | |||
| Exon3 | F: CAACTGTCACCTCTGTCTT | 61.2 | 274 |
| R: GTGGCTATAATTCCTGTGTTAG | |||
| Exon4 | F: ATCGTGTTCCCACTTCAC | 59.6 | 400 |
| R: CAGCACTGCCTTCCCAAA | |||
| Exon5 | F: TGGTGGCTTGTACTGCTA | 56.5 | 288 |
| R: ACACTACTTCAGACCTTCAC | |||
| Exon6 | F: CTTCCACCTTCAACTTCTG | 53.0 | 286 |
| R: TACTTTGGGTTGGGTTCT | |||
| Exon7 | F: CCCCAGAAGGTGACTTAG | 53.0 | 295 |
| R: GGAAGAAGGGTTGGATTT | |||
| Exon8 | F: GTTCAGCCTACCTAAGAGTCCT | 57.8 | 258 |
| R: CCCTCAATGACAGATTCCAG | |||
| Exon9 | F: CAGAGCAAAGCAACCAAG | 60 | 371 |
| R: AAAGCCAGGGGATAGAAT | |||
| Exon10 | F: AAGGCACTGAGCAATGAG | 53.2 | 418 |
| R: TTGGTCCATCAATGAGAACA | |||
| Exon11 | F: GTCCTAAAGGCGTATTCT | 57.8 | 405 |
| R: TAGTGCCATCACAAACAT | |||
| Exon12 | F: TCCGCTTCACCTTCCTGAG | 60 | 482 |
| R: GGCTTGCCATCATCCAGATAT | |||
| Exon13 | F: CTCTCCTTGTAGGGAGCAAG | 55 | 331 |
| R: GTTGCTGCTAATGGAGGATT |