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. 2018 Nov 14;1:192. doi: 10.1038/s42003-018-0181-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — CusF W44 fluorescence quenching and formation of new 490 emission during titration with Cu(I). Increasing aliquots of Cu(II) were added to apo-CusF in the presence of 1 mM sodium ascorbate, in 50 mM HEPES pH 7.5. The main panel shows the decrease in 344 nm emission and the inset shows and expanded view of the concomitant increase in 490 emission (excitation wavelength 295 nm)