Table 1.
Cryo-EM data collection, refinement and validation statistics
| Apo | Sensitized | hTRPV32-APB 1 | hTRPV32-APB 2 | hTRPV32-APB 3 | |
|---|---|---|---|---|---|
| hTRPV3 | hTRPV3 | ||||
| 6MHO | 6MHS | 6MHV | 6MHW | 6MHX | |
| EMD-9115 | EMD-9117 | EMD-9119 | EMD-9120 | EMD-9121 | |
| Data collection and processing | |||||
| Magnification | ×22,500 | ×45,000 | ×36,000 | ||
| Voltage (kV) | 300 | 200 | 200 | ||
| Electron exposure (e- Å–2) | 63 | 67 | 60 | 60 | 60 |
| Defocus range (μm) | −0.5 to −2 | −0.8 to −1.4 | −1.2 to −2 | −1.2 to −2 | −1.2 to −2 |
| Pixel size (Å) | 1.31 | 0.915 | 1.15 | 1.15 | 1.15 |
| Detector | Super-resolution | Counting | Counting | Counting | Counting |
| Total extracted particles (no.) | 361,244 | 559,206 | 897,643 | ||
| Refined particles (no.) | 215,346 | 339,693 | 589,656 | ||
| Reconstruction | |||||
| Final particles (no.) | 27,620 | 44,554 | 141,832 | 61,292 | 84,307 |
| Symmetry imposed | C4 | C4 | C4 | C2 | C2 |
| Nominal resolution (Å) | |||||
| FSC 0.143 (unmasked/masked) | 4.21/3.38 | 4.10/3.24 | 3.83/3.45 | 4.98/3.99 | 4.91/3.99 |
| Map sharpening B factor (Å2) | −73 | −51 | −145 | −152 | −144 |
| Refinement | |||||
| Model composition | |||||
| Non-hydrogen atoms | 17,032 | 17,784 | 18,336 | 17,892 | 16,956 |
| Protein residues | 2404 | 2428 | 2452 | 2452 | 2436 |
| Ligands | 0 | 0 | 0 | 0 | 0 |
| Validation | |||||
| MolProbity score | 1.12 | 1.61 | 1.61 | 1.57 | 1.55 |
| Clashscore | 3 | 6 | 7 | 7 | 7 |
| Poor rotamers (%) | 0 | 0 | 0 | 0.1 | 0 |
| Ramachandran plot | |||||
| Favored (%) | 98 | 96 | 96 | 97 | 97 |
| Allowed (%) | 2 | 4 | 4 | 3 | 3 |
| Disallowed (%) | 0 | 0 | 0 | 0 | 0 |