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. 2018 Nov 14;9(11):1136. doi: 10.1038/s41419-018-1184-7

Fig. 1. Suppressed osteoblastic differentiation of hBMSCs by overexpression of STAT5A.

Fig. 1

a Protein levels of STAT5A, STAT5B, DLX5, and RUNX2 during osteogenesis of hBMSCs. b Alizarin Red S staining demonstrating the effect of STAT5 overexpression on the osteogenesis at day 14. Scale bar, 60 μm. c Quantification of Alizarin Red S staining. d Western blot analysis of protein expression of osteogenic master genes, DLX5 and Runx2 after STAT5 overexpression. GAPDH was used as a control. e Quantification of DLX5 and RUNX2 protein levels. f Gene expression of DLX5 and expression of downstream genes of DLX5 after 4 days with STAT5 overexpression in hBMSCs using real-time PCR. All mRNA and protein levels were normalized with GAPDH. Each experiment was performed in triplicate (n = 3). All error bars indicate ± SEM. *P < 0.01; **P < 0.01; ***P < 0.001