Figure 4.

Enzyme modified LDL (ELDL) upregulates phosphate induced calcification in cultured human coronary artery smooth muscle cells. (A) HCASMC cultured in phosphate containing medium (as indicated) with either control BSA or ELDL as indicated. Calcium phosphate deposits were stained with Alizarin Red. (B) Quantification of Ca² deposits. (C) mRNA levels of selected osteoblastic genes in HCASMC incubated for 24 h with either 10 μg/mL ELDL or control BSA with 1 mM inorganic phosphate. Reproduced with permission from Macmillian Publishers Ltd. (62). *p < 0.05; **p < 0.01.