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. 2018 Nov 14;8:16786. doi: 10.1038/s41598-018-34884-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A map of the constructed vector. (A) Schematic of the BaPDSs gene fragment indicating the sgRNA target site and sequence. indicates exon. indicates intron. (B) Gel electrophoresis of the recombinant plasmid of pSG-BaPDS and pCC. M, DL15000 marker. (C) Structure of the CRISPR/Cas9 binary vectors for Chinese kale transformation. The Cas9 cassette was driven by the 35S promoter, while sgRNA was controlled by the hU6 promoter. The 3 × Flag is a label which can be used to identify the transgenic plants. NLS, nuclear localization sequence.

Inline graphic — A map of the constructed vector. (A) Schematic of the BaPDSs gene fragment indicating the sgRNA target site and sequence. indicates exon. indicates intron. (B) Gel electrophoresis of the recombinant plasmid of pSG-BaPDS and pCC. M, DL15000 marker. (C) Structure of the CRISPR/Cas9 binary vectors for Chinese kale transformation. The Cas9 cassette was driven by the 35S promoter, while sgRNA was controlled by the hU6 promoter. The 3 × Flag is a label which can be used to identify the transgenic plants. NLS, nuclear localization sequence.