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. 2018 Nov 14;8:16786. doi: 10.1038/s41598-018-34884-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PCR detection of the hygromycin-resistant gene for the estimation of transformation efficiency. M: DL2000 maker; V1, V2: empty vector was used as a positive control; P: plasmid containing the vector and sgRNA also was used as a positive control; H,W: H₂O and the gDNA of the wild-type was used as a negative control; 1–75: indicates the resistant plant line number. The arrow points to the aim band.