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. 2018 Nov 14;8:16786. doi: 10.1038/s41598-018-34884-9

Figure 3.

Figure 3

PCR detection of the hygromycin-resistant gene for the estimation of transformation efficiency. M: DL2000 maker; V1, V2: empty vector was used as a positive control; P: plasmid containing the vector and sgRNA also was used as a positive control; H,W: H2O and the gDNA of the wild-type was used as a negative control; 1–75: indicates the resistant plant line number. The arrow points to the aim band.