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. 2018 Nov 14;8:16786. doi: 10.1038/s41598-018-34884-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CRISPR/Cas9 system-induced mutation detection in Chinese kale mutants. (A) BaPDS1 mutations at the target site; (B) BaPDS1 mutations within introns; (C) BaPDS2 mutations at the target site; (D) BaPDS2 mutations within introns. The target sequence is indicated in blue, the PAM sequence (NGG) is underlined in red, mutated bases are indicated in red font, the short line represents the deletion base, and the asterisks indicate the spacing between bases. i #, # number of base insertions. r #, # number of base replacements. d #, # number of base deletions. The number of identical mutations is indicated in parentheses. Mn contains strains M4, M7, M8, M9, M10, M11, M12, M13, M20, M23, M26, M31, M32, M33, M36, M37, M38, M41, M42, M44, M45, M48, M50, M55, M57, M60, M65, M70, M72, M73.