Table 5.
Effect of cell density on plasmid curing efficiency of lawsone.
| Plasmid | Curing efficiency (%) at |
|||
|---|---|---|---|---|
| 104 (cells/ml) | 105 (cells/ml) | 106 (cells/ml) | 107 (cells/ml) | |
| E. coli (pBR 322) | 31.3 ± 1.8 | 16.5 ± 2.1 | 2 ± 0 | ND |
| E. coli (pRK 2013) | 20 ± 1.4 | 9.3 ± 2.5 | 4.5 ± 0.7 | 9 ± 0 |
| A. baumannii (pUPI 281) | 12.5 ± 2.1 | 15.5 ± 2.1 | 15 ± 0 | 5.8 ± 1.0 |
Above desired number of cells were incubated in Luria broth in the presence of 512 μg of lawsone/ml at 37°C for 24 h. Number of clones tested – 300. ND, none of the 300 colonies tested showed curing of plasmid.