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. 2018 Sep 28;4(4):341–350. doi: 10.1002/vms3.123

Table 1.

Oligonucleotides used for qPCR to measure relative expression levels of bovine PAG‐genes during pregnancy

Gene Accession Number Primer Sequence (5′‐3′) Product size (bp)
cDNA gDNA
boPAG‐1 NM_174411.2 for TAGGCTCATCGGTGCCATAC 116 203
rev GACCTGGCACTGGGTAGTTG
boPAG‐2 NM_176614.1 for TCCTGGAGGAACAAGCTTACA 149 1111
rev TGAGCCTGTGTCAAAGACGA
boPAG‐8 NM_176619.3 for GACACCGGCTCATCTGACTT 139 229
rev CAGAGCCGTAGACGAGTTCA
boPAG‐9 NM_176620.2 for TGAAGTGGATTGTGCTCCTC 149 1228
rev CTGGGACAGTCTGTAAGGATGC
boPAG‐10 NM_176621.3 for TTGAGCAGTCAGAAAGAGAACG 137
rev TTCATGGAGATGCTGTCTATGTTT
boPAG‐11 NM_176623.2 for GAAGATGACAGGAGGCAGGATAC 138 221
rev GTGGATACCGGGACATCACT
boPAG‐12 NM_176622.1 for TCCTGGAAGAACGAGCTTACA 149 1113
rev TGAGCCTGTGTCAAAGACGA
boPAG‐21 NM_176630.2 for TACAGGCTCATCTGACTTTTGG 141 240
rev CTCCCAGATCCATAGGTGATGC
CDH1 NM_001002763.1 for TGCCCAGAAAATGAGAAAGG 99 225
rev TTGGCCAGTGATGCTGTAGA
YHWAG NM_174793.2 for AGCACATGCAGCCCACTC 121
rev TCGTCGAAGGCGGTCTTG