Table III.
Primer sequences of mRNA used for reverse transcription-quantitative polymerase chain reaction.
| Primer ID | Primer sequences | Annealing temperature (°C) | Length (base pairs) |
|---|---|---|---|
| COL1A1 | F:AGACATCCCACCAATCACCT | 60 | 118 |
| R:GTCATCGCACAACACCTTG | |||
| ASXL1 | F:CCTACTGTCCTCCCAAACC | 60 | 118 |
| R:CTCCTCATCATCACTTTCCC | |||
| HIPK3 | F:GTTGTGATACGGTGGATGG | 60 | 135 |
| R:CTGGCTTGGTTTCTGTGTC | |||
| CTGF | F:TACCAATGACAACGCCTCCTG | 60 | 208 |
| R:GCCGTCGGTACATACTCCACA | |||
| TGFb3 | F:GCTACTATGCCAACTTCTGCTCA | 60 | 224 |
| R:GCTACATTTACAAGACTTCACCACC | |||
| GAPDH | F:GGACCTGACCTGCCGTCTAG | 60 | 100 |
| R:GTAGCCCAGGATGCCCTTGA |
ASXL1, ASXL transcriptional regulator 1; COL1A1, collagen type I α1 chain; CTGF, connective tissue growth factor; F, forward; HIPK3, homeodomain interacting protein kinase 3; R, reverse; TGF β3, transforming growth factor β3.