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. 2018 Oct 17;18(6):5572–5578. doi: 10.3892/mmr.2018.9564

Figure 4.

Figure 4.

Zey regulates extracellular matrix-associated factors in DU145 cells. (A) DU145 cells exposed to Zey at 0 (control), 10 (Zey1), 20 (Zey2) and 40 (Zey3) µmol/l for 48 h. Expression levels of MMP-2 and MMP-9 were detected using an ELISA. (B) mRNA expressions of MMP-2, MMP-9, TIMP-1, FN-1 and collagen-1 were measured by a reverse transcription-quantitative polymerase chain reaction assay. (C) Protein expression of MMP-2, MMP-9, TIMP-1, FN-1 and collagen-1 were measured by western blot analysis and normalized to β-actin expression. Gray value was evaluated and quantified using Quantity One software. ^P<0.05, ^^P<0.01, ^^^P<0.001 vs. respective control. Zey, Zeylenone; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinases; FN-1, fibronectin.