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. 2018 Oct 24;18(6):5445–5454. doi: 10.3892/mmr.2018.9586

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Copyright: © Su et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Renal cell apoptosis in rats from each group. (A) Representative images of protein expression of Bcl-2, caspase-9, caspase-3 and cleaved caspase-3, obtained by western blot analysis. Relative protein expression levels of (B) Bcl-2, (C) caspase-9, (D) caspase-3 and (E) cleaved caspase-3 were determined by quantitative analysis. (F) Representative images of cell apoptosis in the kidney tissues of rats (TUNEL staining; ×200 magnification). (G) Percentage of apoptotic cells in each group (quantitative analysis). The results are presented as the mean ± standard deviation. n=10/group. *P<0.05 CIR vs. CON; ^#P<0.05 RAPA vs. CIR. CON, control group; CIR, cerebral ischemia-reperfusion; RAPA, rapamycin pre-treatment prior to CIR; Bcl-2, B-cell lymphoma 2.