Table I.
Primers used for reverse transcription-quantitative polymerase chain reaction.
| Gene | Forward primers | Reverse primers | NCBI Accession no.a |
|---|---|---|---|
| GAPDH | 5′-AAGCTCATTTCCTGGTATGACA-3′ | 5′-TCTTACTCCTTGGAGGCCATGT-3′ | NM_017008.4 |
| Scx | 5′-AGAACACCCAGCCCAAACA-3′ | 5′-CGGTCTTTGCTCAACTTTCT-3′ | NM_001130508 |
| Col1 | 5′-GTGCTAAGGGTGAAGCTGGT-3′ | 5′-CATCAGCACCAGGGTTTCCAG-3′ | NM_053304 |
| Tnmd | 5′-GTCACATTCTAAATGCAGAAG-3′ | 5′-CTCCCCCAAAACAGGACAAT-3′ | NM_022290 |
| Col3 | 5′-CTGGAGATAAGGGTGAAGGT-3′ | 5′-GAGGGCCTCCTTCACCTTTCT-3′ | NM_032085 |
| Mkx | 5′-CTATCGCACAGGTAAGCCCA-3′ | 5′-CCCACGTATCAGTTTCTCCCA-3′ | XM_017600733 |
| Egr1 | 5′-AACAACCCTACGAGCACCTG-3′ | 5′-ACCAGCGCCTTCTCGTTATT-3′ | NM_012551 |
| Fmod | 5′-CCCGTGATTGTCCCCAAGAA-3′ | 5′-CAGGTACTTGAGGTTGCGGT-3′ | NM_080698 |
| Lum | 5′-GCTTCACCGGGCTTCAATAC-3′ | 5′-AAATGAGTTTCCAGGCACGC-3′ | NM_031050 |
| Dcn | 5′-CCTAAAGGAGCTGCCCGAAA-3′ | 5′-GCCGCCCAGTTCTATGACAA-3′ | NM_024129 |
| Bgn | 5′-CTGCATTGAGATGGGTGGGA-3′ | 5′-GGTAGTTGAGCTTCAGGCCA-3′ | NM_017087 |
a
NCBI Accession nos. available at https://www.ncbi.nlm.nih.gov/. Scx, scleraxis; Col1, collagen type 1; Tnmd, tenomodulin; Col3, collagen type 3; Mkx, mohawk; Egr1, early growth response gene 1; Fmod, fibromodulin; Lum, lumican; Dcn, decorin; Bgn, biglycan.