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Primer sequences used in reverse transcription-quantitative polymerase chain reaction.
| Gene | Direction | Primer sequence (5′-3′) | Primer length (bp) | Product length (bp) | Annealing temperature (°C) |
|---|---|---|---|---|---|
| Bcl-2 | Forward | GGCATCTTCTCCTTCCAGC | 19 | 265 | 58 |
| Reverse | AGAGTTCCTCCACCACCGT | 19 | |||
| GAPDH | Forward | GCAAGTTCAACGGCACAG | 18 | 141 | 58 |
| Reverse | CGCCAGTAGACTCCACGAC | 19 |
Bcl-2, BCL2 apoptosis regulator.
