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. 2018 Oct 25;18(6):5427–5436. doi: 10.3892/mmr.2018.9599

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of AG on IL-1β-induced extracellular matrix degradation in human NP cells. NP cells were stimulated by IL-1β with or without AG treatment. Immunofluorescence images of (A) collagen-II and (B) aggrecan expression following treatment (magnification, ×400). (C) The protein expression levels of aggrecan and collagen were determined western blot analysis. (D) Quantification of collagen levels. (E) Quantification of aggrecan levels. The values are presented as the mean ± standard deviation of 3 independent experiments. *P<0.05 vs. control group; ^#P<0.05 vs. IL-1β group. AG, andrographolide; IL-1β, interleukin 1β; NP, nucleus pulposus.