Figure 1.

Effect of LPS and RANKL on osteoclast formation in Raw264.7 cell cultures. (A) Raw264.7 cells were treated with 30 ng/ml RANKL or 100 ng/ml LPS or a combination of RANKL and LPS for 3 days. Cells were fixed and stained for TRAP. TRAP+ cells with >3 nuclei were classified as osteoclasts. Magnification, ×200. (B) Bars represent the mean ± standard error of the mean. *P<0.05 and ***P<0.001. LPS, lipopolysaccharide; RANKL, receptor activator of nuclear factor κβ ligand; TRAP, tartrate-resistant acid phosphatase; MNCs, multinuclear cells; Con, control.