Figure 2.

LPS highly induces the cytokine production in osteoclastogenesis. Raw264.7 cells treated with 30 ng/ml RANKL and 100 ng/ml of LPS for 3 days. A reverse transcription-quantitative polymerase chain reaction was performed for the detection of IL-1β, IL-6 and TNF-α mRNA levels. The figure presents the relative expression levels of target mRNA normalized to endogenous GAPDH mRNA levels. Bars represent the mean ± standard error of the mean. ***P<0.001. LPS, lipopolysaccharide; RANKL, receptor activator of nuclear factor κβ ligand; IL, interleukin; TNF-α, tumor necrosis factor-α; NS, non-significant; Con, control.