Figure 3.

LPS highly induces miR-146a expression in osteoclastogenesis in Raw264.7 cells. (A) Cells were treated with 30 ng/ml RANKL and 100 ng/ml of LPS for 24, 48 and 72 h. (B) Cells were treated with 100 ng/ml LPS, or a combination of 30 ng/ml RANKL and 100 ng/ml LPS for 24 h. Total RNA was extracted for the detection of miR-146a expression using the Taqman method. Bars represent the mean ± standard error of the mean. **P<0.01, ***P<0.001. LPS, lipopolysaccharide; miR, microRNA; RANKL, receptor activator of nuclear factor κβ ligand; Con, control; NS, not significant.