Table I.
Primer sequences used for reverse transcription-quantitative polymerase chain reaction.
| Gene | Primer sequences (5′→3′) |
|---|---|
| FAK | F: ATCCCACACATCTTGCTGACTT |
| R: GCATTCCTTTTCTGTCCTTGTC | |
| PI3K | F: CCCTGCTCATCAACTAGGAAACC |
| R: TTGCCGTAAATCATCCCCATT | |
| AKT | F: TGCCCTTCTACAACCAGGAC |
| R: ACACGATACCGGCAAAGAAG | |
| Snail | F: TCCTTCGTCCTTCTCCTCTACTT |
| R: TGTTGCAGTATTTGCAGTTGAAG | |
| Slug | F: AGATGCATATTCGGACCCACA |
| R: CCTCATGTTTGTGCAGGAGAG | |
| Twist1 | F: CAAGTCTGCAGCTCTCGCCA |
| R: CCAACGGCTGGCGCACAC | |
| E-cadherin | F: ATTGCTCACATTTCCCAACTCC |
| R: CTCTGTCACCTTCAGCCATCCT | |
| Cytokeratin | F: ACATCGAGATCGCCACCTAC |
| R: CCAGAGCCAAAGCTGGAG | |
| N-cadherin | F: AGCCAACCTTAACTGAGGAGT |
| R: GGCAAGTTGATTGGAGGGATG | |
| Vimentin | F: TACAGGAAGCTGCTGGAAGG |
| R: ACCAGAGGGAGTGAATCCAG | |
| GAPDH | F: CTCCTCCACCTTTGACGCTG |
| R: TCCTCTTGTGCTCTTGCTGG |
AKT, protein kinase B; E, epithelial; forward; FAK, focal adhesion kinase; N, neural; PI3K, phosphoinositide 3-kinase; R, reverse; Twist1, twist-related protein 1.