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. 2018 Oct 10;18(6):4821–4830. doi: 10.3892/mmr.2018.9544

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Copyright: © Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Formononetin rescues I/R-induced cellular apoptosis in isolated heart tissue. (A) TUNEL staining detected cardiomyocyte apoptosis. Nuclei with brown staining indicate TUNEL-positive cells. Arrows indicate apoptotic cells (×400 magnification). (B) Statistical analysis of the results of the TUNEL assay. (C) Western blot analysis of cleaved caspase-3 (19 kDa), Cyt-c (14 kDa) and Bcl-2 (26 kDa). The intensity of each band was quantified and normalized to actin: (D) caspase 3; (E) Cyt-c; and (F) Bcl-2. All data were from three independent experiments.*P<0.05, **P<0.01 and ***P<0.001. TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling; I/R, ischemia/reperfusion; Form, treated with formononetin; PC, preconditioning; Cyt-c, cytochrome c; Bcl-2, apoptosis regulator Bcl-2.

Figure 2. — Formononetin rescues I/R-induced cellular apoptosis in isolated heart tissue. (A) TUNEL staining detected cardiomyocyte apoptosis. Nuclei with brown staining indicate TUNEL-positive cells. Arrows indicate apoptotic cells (×400 magnification). (B) Statistical analysis of the results of the TUNEL assay. (C) Western blot analysis of cleaved caspase-3 (19 kDa), Cyt-c (14 kDa) and Bcl-2 (26 kDa). The intensity of each band was quantified and normalized to actin: (D) caspase 3; (E) Cyt-c; and (F) Bcl-2. All data were from three independent experiments.*P<0.05, **P<0.01 and ***P<0.001. TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling; I/R, ischemia/reperfusion; Form, treated with formononetin; PC, preconditioning; Cyt-c, cytochrome c; Bcl-2, apoptosis regulator Bcl-2.