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. 2018 Sep 20;18(6):4831–4838. doi: 10.3892/mmr.2018.9502

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — TIMP2 is negatively regulated by miR-939. (A) Successful transfection efficiency was determined by reverse transcription-quantitative polymerase chain reaction. **P<0.01. (B) In the prediction programs miRDB and TargetScan Human, the 3′-UTR of TIMP2 was indicated to have a binding site for miR-939. (C) Relative luciferase activity was evaluated. 1, pGL3-TIMP2; 2, pGL3-TIMP2+miR-939 mimics; 3, pGL3-TIMP2+NC; 4, pGL3-TIMP2 Mutant; 5, pGL3-TIMP2 Mutant+miR-939 mimics; 6, pGL3-TIMP2 Mutant+NC. **P<0.01 vs. group 1. (D) The relative protein expression of TIMP2 in H1299 (left) and SPCA1 (right) cells was detected by western blot analysis. miR, microRNA; NC, negative control; TIMP2, TIMP metallopeptidase inhibitor 2; UTR, untranslated region; WT, wild-type.