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. 2018 Oct 30;18(6):5552–5562. doi: 10.3892/mmr.2018.9613

Figure 2.

Figure 2.

UBA2 expression levels in colorectal cancer cell lines. (A) UBA2 mRNA expression levels in colon cancer cell lines were detected by RT-qPCR. (B) UBA2 mRNA expression levels in RKO cells transfected with UBA2-siRNA or NC-siRNA. (C) UBA2 protein expression levels in RKO cells transfected with UBA2-siRNA or NC-siRNA were detected by western blotting. (D) UBA2 mRNA expression levels in HCT116 cells transfected with UBA2-siRNA or NC-siRNA were detected by RT-qPCR. (E) UBA2 protein expression levels in HCT116 cells transfected with UBA2-siRNA or NC-siRNA were detected by western blotting. All experiments were performed three times in triplicate. NC, negative control; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; siRNA, small interfering; UBA2, ubiquitin like modifier activating enzyme 2.