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. 2018 Oct 30;18(6):5552–5562. doi: 10.3892/mmr.2018.9613

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Copyright: © He et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effects of UBA2 on the cell cycle and apoptosis. (A and B) Cell cycle analysis by flow cytometry in RKO cells transfected with (A) NC-siRNA and (B) UBA2-siRNA. (C) Relative quantity of cells in G0/G1, S and G2/M phases in the RKO cells transfected with UBA2-siRNA or NC-siRNA. (D and E) Cell cycle analysis by flow cytometry in HCT116 cells transfected with (D) NC-siRNA and (E) UBA2-siRNA. (F) Relative quantity of cells in G1/G1, S and G2/M phases in the HCT116 cells transfected with UBA2-siRNA or NC-siRNA. (G-J) Apoptosis analysis by flow cytometry in (G) RKO cells transfected with NC-siRNA, (H) RKO cells transfected with UBA2-siRNA, (I) HCT116 cells transfected with NC-siRNA, and (J) HCT116 cells transfected with UBA2-siRNA. (K and L) Apoptotic rates calculated from data in RKO cells and HCT116 cells. *P<0.05, **P<0.01 vs. the NC-siRNA. All experiments were performed three times in triplicate.