Analysis of correlation between Sel1L‐Hrd1 ERAD and Crebh‐Fgf21 during fasting‐feeding (A–D) and growth (E–H).
-
A
Western blot analysis of hepatic Sel1L‐Hrd1 ERAD and Crebh of nuclear (Nuc) and cytosolic (Cyto) fractions from 10‐week‐old Sel1L
f/f and Sel1L
Alb mice under overnight fasted or fed states.
-
B
Quantitation of levels of Sel1L/Hrd1/Crebh proteins and Fgf21 mRNA in the livers of 10‐week‐old Sel1L
f/f mice.
-
C
Quantitation of total Crebh (Crebh+Crebh‐N) protein levels in the livers of 10‐week‐old Sel1L
f/f and Sel1L
Alb mice.
-
D
Serum Fgf21 levels in 10‐week‐old Sel1L
f/f and Sel1L
Alb mice.
-
E–H
Similar to (A–D) with the exception that these experiments were done with the livers from Sel1L
f/f and Sel1L
Alb mice at 3, 9, and 24 weeks of age.
Data information: Hsp90 and lamin, loading controls for cytosolic and nuclear fractions. Values are mean ± SEM; **
P < 0.01 by two‐way ANOVA analysis.
n = 3–4 per group, 2 independent repeats.