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. 2018 Nov 2;37(22):e99277. doi: 10.15252/embj.201899277

Figure 7. Sel1L‐Hrd1 ERAD represses Crebh and Fgf21 under fasting‐feeding and growth.

Figure 7

Analysis of correlation between Sel1L‐Hrd1 ERAD and Crebh‐Fgf21 during fasting‐feeding (A–D) and growth (E–H).
  • A
    Western blot analysis of hepatic Sel1L‐Hrd1 ERAD and Crebh of nuclear (Nuc) and cytosolic (Cyto) fractions from 10‐week‐old Sel1L f/f and Sel1L Alb mice under overnight fasted or fed states.
  • B
    Quantitation of levels of Sel1L/Hrd1/Crebh proteins and Fgf21 mRNA in the livers of 10‐week‐old Sel1L f/f mice.
  • C
    Quantitation of total Crebh (Crebh+Crebh‐N) protein levels in the livers of 10‐week‐old Sel1L f/f and Sel1L Alb mice.
  • D
    Serum Fgf21 levels in 10‐week‐old Sel1L f/f and Sel1L Alb mice.
  • E–H
    Similar to (A–D) with the exception that these experiments were done with the livers from Sel1L f/f and Sel1L Alb mice at 3, 9, and 24 weeks of age.
Data information: Hsp90 and lamin, loading controls for cytosolic and nuclear fractions. Values are mean ± SEM; **P < 0.01 by two‐way ANOVA analysis. n = 3–4 per group, 2 independent repeats.