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. 2018 Nov 5;11:1179066018809091. doi: 10.1177/1179066018809091

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Immunohistochemical staining of active caspase-8 in 4-µm-thick paraffin-embedded cerebellar sections. (A) From control. (B) From experimental. Scale bar shown in (B) applies to both images (A and B) in the figure. Hematoxylin-stained nuclei help to differentiate the molecular (ML), Purkinje cell (PC), and granule cell (GL) layers. (A) Active caspase-8 immunostaining is hardly observed in the control cerebellum. (B) Active caspase-8 immunoreactivity is strong in PCs (such as those at the tips of the black arrows) and granule neurons (such as those at the tips of the white arrows) from the experimental group. (C) The level of active caspase-8 expression increased significantly in the experimental cerebella compared to that in the control group (P < .01*). Cr., control; Exp., experimental.