Figure 1.

Flowering time and gene expression levels in Ubi::HvFT3 transgenic lines under long-day and short-day conditions. Flowering time and expression levels of HvFT3, VRN-H1, and HvFT1 were estimated in Ubi::HvFT3 transgenic lines (N1, N2, N4, N16, and N23), a null segregant line (Null), and Golden Promise (wild type [WT]) under long day (LD; 16 h light, red) and short day (SD; 8 h light, black) conditions. Each column represents the average (A) flowering time, (B) HvFT3 expression levels, (C) VRN-H1 expression levels, and (D) HvFT1 expression levels. Flowering time was measured in days from germination until heading. For A, B, C, and D, four to 15 plants were used as biological replicates. Error bars, standard deviation. Expression analysis was performed on leaf samples collected 2 h before the end of the light period at day 5 after germination. Expression values were normalized to HvActin. NF, Did not flower; ND, not detected.