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. 2018 Sep 18;178(3):1045–1064. doi: 10.1104/pp.18.00584

Figure 4.

Figure 4.

Carbohydrate analysis of total mucilage extracted with water. A, Overview of the total mucilage extraction, which removes all polysaccharides from the seed surface that can be stained with RR. B and C, Monosaccharide composition of total mucilage extracted from seeds. Data show means + sd of four biological replicates per genotype. Significant changes from the wild type and between mutants are indicated by different red letters (Student’s t test, P < 0.05). The monosaccharide composition of the lines shown in B is provided in Supplemental Table S2, along with the data for gaut11-4, gaut10-1, and gaut14-1 mutants. D and E, Monosaccharide composition of the alcohol-insoluble residue (AIR) isolated from total mucilage extracts, following EDTA pretreatment, and the remaining seeds. Data show means + sd of three biological replicates. Asterisks indicate significant changes relative to the wild type (Student’s t test, P < 0.05).