Table 1. Glycosyl linkages in total mucilage extracted with water.
Linkage | Wild Type | muci70-1 | gaut11-3 |
---|---|---|---|
Rha | |||
t-Rha | 0.113 ± 0.000 | 0.175 ± 0.069 | 0.204 ± 0.093 |
2-Rha | 9.082 ± 0.048 | 1.949 ± 0.141 | 6.277 ± 0.319 |
2,3-Rha | 0.220 ± 0.025 | 0.066 ± 0.013 | 0.156 ± 0.031 |
2,4-Rha | 0.093 ± 0.065 | 0.051 ± 0.010 | 0.095 ± 0.007 |
Ara | |||
t-Ara | 0.056 ± 0.007 | 0.030 ± 0.009 | 0.042 ± 0.014 |
5-Ara | 0.069 ± 0.000 | 0.021 ± 0.008 | 0.047 ± 0.009 |
3-Ara | 0.167 ± 0.012 | 0.114 ± 0.018 | 0.208 ± 0.021 |
Gal | |||
t-Gal | 0.153 ± 0.012 | 0.288 ± 0.071 | 0.313 ± 0.034 |
2-Gal | 0.072 ± 0.016 | 0.127 ± 0.010 | 0.270 ± 0.024 |
4-Gal | 0.097 ± 0.001 | 0.052 ± 0.007 | 0.108 ± 0.008 |
6-Gal | 0.024 ± 0.002 | 0.021 ± 0.007 | 0.035 ± 0.006 |
2,4-Gal | 0.036 ± 0.008 | 0.026 ± 0.003 | 0.045 ± 0.004 |
4,6-Gal | 0.092 ± 0.001 | 0.075 ± 0.026 | 0.168 ± 0.009 |
Glc | |||
t-Glc | 0.013 ± 0.009 | 0.014 ± 0.007 | 0.029 ± 0.009 |
4-Glc | 0.246 ± 0.037 | 0.373 ± 0.052 | 0.767 ± 0.089 |
3,4-Glc | 0.014 ± 0.003 | 0.014 ± 0.003 | 0.036 ± 0.010 |
4,6-Glc | 0.029 ± 0.003 | 0.037 ± 0.013 | 0.101 ± 0.025 |
Xyl | |||
t-Xyl | 0.172 ± 0.005 | 0.335 ± 0.062 | 0.108 ± 0.014 |
4-Xyl | 0.640 ± 0.034 | 1.070 ± 0.090 | 0.361 ± 0.009 |
2,4-Xyl | 0.258 ± 0.018 | 0.372 ± 0.026 | 0.125 ± 0.013 |
Man | |||
4-Man | 0.061 ± 0.002 | 0.092 ± 0.012 | 0.157 ± 0.013 |
4,6-Man | 0.159 ± 0.018 | 0.228 ± 0.044 | 0.562 ± 0.041 |
GalA | |||
t-GalA | 0.075 ± 0.002 | 0.049 ± 0.007 | 0.096 ± 0.028 |
4-GalA | 12.175 ± 0.647 | 3.793 ± 0.394 | 9.252 ± 0.625 |
2,4-GalA | 0.128 ± 0.003 | 0.060 ± 0.018 | 0.085 ± 0.007 |
4,6-GalA | 0.165 ± 0.001 | 0.053 ± 0.007 | 0.146 ± 0.033 |