Figure 6.

Effects of RNA interference and overexpression of GmINS1 on infection cell development in nodules. A, Toluidine Blue-stained nodule cross sections of Ri and OX lines of GmINS1. IV to VI are magnified images of the respective images I to III showing the infection zone. Bars = 200 µm (I–III) and 100 µm (IV–VI). B, Number of infection cells. C, Surface area of 100 infection cells. Soybean transgenic composite plants inoculated with rhizobia were grown in sand culture irrigated with low-N nutrient solution for 30 d. Each bar represents the mean of five biological replicates with se. Five nodules were selected and measured from each replicate. Different letters indicate significant differences between Ri or OX lines and Ev control plants for the same trait in a two-way ANOVA test (P < 0.05).