Figure 8.

SES1 has chaperone activity. A, Holdase chaperone activity of SES1 with the model substrate MDH. MDH (300 μm) was incubated without or with recombinant GST-SES1 at 45°C for the indicated times. GST was used as a negative control. The means were calculated from three independent replicates. B, GFP fluorescence of p35S::secGFP in ses1-2 and wild-type (WT) transgenic lines was imaged with a high-resolution laser confocal microscope at 488 nm. Bars = 100 µm. C, GFP and RFP fluorescence of transgenic lines containing secGFP and HDEL-RFP in the ses1-2 background was imaged with a high-resolution laser confocal microscope at 488 and 610 nm, respectively. Bars = 20 µm. D, Analysis of total and ER-localized secGFP in wild-type and ses1-2 seedlings. Protein was extracted from 10-d-old seedlings.