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. 2018 Oct 5;178(3):989–1001. doi: 10.1104/pp.18.00828

Figure 3.

Figure 3.

amiRNA designed by the new strategy for silencing a specific alternatively spliced transcript. A, Designing amiRNA for silencing Arabidopsis SR45.1 but not SR45.2 by targeting SR45.1-specific sequence. B, Specificity inspection by WMD3-Target Search identifies SR45.1-specific amiRNA candidates. amiR-5 (red) was identified by WMD3-Target Search as an SR45.1-specific amiRNA and was renamed amiR-SR45.1-m1 for transgenic expression. Note that amiR-6 and amiR-8, despite being designated as SR45.1-specific amiRNA candidates, were abandoned because they share overlapping target sequences with amiR-5. C, Transgenic SR45.1-silencing plants exhibit a similar flower petal phenotype to the sr45-1 null mutant. Bars = 0.5 mm. D, Transgenic SR45.1-silencing plants are different from the sr45-1 null mutant regarding the root growth phenotype. Bar = 5 mm. sr45-1,null mutant of both SR45.1 and SR45.2; WT, wild type.