Figure 5.

Deg5 and Deg8 only partially complement the deg1 mutant. The wild type (WT), deg1 mutant, and deg1 mutant complemented with Deg1-His (∆1D1H.1, ∆1D1H.2), Deg5-His (∆1D5H.1), Deg8-His (∆1D8H.1), or Deg5-His/Deg8-His (∆1D58H.1) were germinated on MS agar with or without antibiotic selection (Supplemental Table S4) for 8 d, then seedlings were transferred to soil and grown under optimal conditions for 13 d (growth light [GrL]: ∼160 µmol photons m⁻² s⁻¹, 18 h of light/6 h of dark, 22°C/18°C). Experimental plants were either transferred to a greenhouse at 22°C or 34°C with natural diurnal fluctuating light intensity (daylight [DL]: maximum of ∼1,600 μmol photons m⁻² s⁻¹ at midday) or kept under growth light conditions for an additional 7 d. A, Immunoblot analysis of protein content in the various genotypes (genotypes are indicated above the immunoblots) following 20 d of growth under optimal conditions (Supplemental Fig. S4A). Total protein was extracted from rosette leaves, separated by SDS-PAGE, and detected with the specific antibodies indicated on the right. The migration of molecular mass (MW) markers is indicated on the left. Five micrograms of chlorophyll was loaded in each lane. Ponceau staining of membranes was used as a loading control. B, Chlorophyll (Chl) content in plants grown at 22°C under growth light (blue bars) and daylight (orange bars) conditions (representative images of plants are shown in Supplemental Fig. S4, A and B, respectively). Values are means ± se of 16 replicates (four plants per genotype, four rosette leaves per plant). Data were analyzed using one-way ANOVA for each lighting condition. Shared letters indicate no statistically significant difference. Note that letters are applicable only within individual lighting treatments. C, Representative images of the wild type, deg1 mutant, and deg1 complemented plants (genotypes are indicated adjacent to the images) after 7 d of growth at 34°C in the greenhouse under daylight conditions.