Figure 5.

VAMP711 is involved in drought stress response. A. Detached rosette leaves from Col-0, vamp711-6, vamp711-7, and OE-VAMP711-15/27. Photographs were taken at 0 (left) and 3 h (right). B, Water-loss measurement of detached leaves. Col-0, vamp711-6, vamp711-7, and OE-VAMP711-15/27 were grown in short-day conditions for 5 weeks. Fresh weight of detached leaves was monitored at the indicated times. The experiment was repeated three times independently. C, Stomatal aperture assay. Detached rosette leaves from Col-0, vamp711-6, and vamp711-7 were incubated in KCl buffer for about 2 h and then transferred in a buffer containing 10 μm of ABA for 2 h. Error bars represent sd (stomata number, 70); Student’s t test was used to assess statistical significance (P ≤ 0.05). Significant differences were represented by different lowercase letters. The experiment was repeated three times independently.