Figure 2.

Expression patterns of genes involved in chloroplast-related functions. A, Expression of genes involved in photosynthesis (i.e. encoding subunits of the thylakoid electron transport chain, tetrapyrrole biosynthesis, plastid ribosomal protein, RNA metabolism, and chlorophyll degradation). Each line describes the expression pattern of a single gene; the core values for each cluster are plotted in black. The percentage of expression was defined as the ratio between expression values at every single time point and the sum of the values of all the time points, given as 100%. The expression values of all the genes are reported in Supplemental Table S9. B, Immunoblot analyses of total protein extracts from 3-, 6-, 9-, and 12-DPA developing Col-0 siliques, whose seeds have been removed manually. Total protein extracts obtained from identical silique fresh weight were fractionated by SDS-PAGE (12% polyacrylamide) and transferred on polyvinylidene difluoride filters. Filters were then immunodecorated using antibodies specific for LhcA2, LhcB3, PsbO, PRPS1, PRPL4, PAO, and Histone H3 proteins. A replicate SDS-PAGE gel stained with Coomassie Brilliant Blue (C.B.B.) is shown as a loading control. One out of three immunoblots for each antibody is shown.