Figure 3.

Promoter-driven GUS expression analysis to validate RNAseq data. The expression of the promoters of FT, LOX3, LOX4, ABI5, LOX2, and ARR15 fused to the GUS reporter gene was evaluated on the basis of UidA enzymatic activity. Silique images are representative of analyses performed on 10 siliques for each stage and transgenic line. At right, the level of expression of each gene (according to the RNAseq data reported in Supplemental Table S10) is shown. The images shown are composite images. Bars = 1 mm.