Figure 4.

Expression patterns of selected genes by RT-qPCR analysis and RNAseq. The x axis indicates the four selected time points, while the y axis shows the mRNA levels: the scale at left indicates gene expression level based on RT-qPCR, and the scale at right is based on RNAseq. The blue lines correspond to RT-qPCR and the orange ones to RNAseq. RT-qPCR was performed on cDNA extracted from Col-0 siliques devoid of seeds and collected at the same time points chosen for RNAseq. RT-qPCR was conducted in triplicate using PP2A and UBC9 as internal reference genes (Supplemental Table S11). The graphs depict the results obtained using PP2A as the internal reference gene; identical results were obtained using UBC9 as the internal reference gene. Error bars represent the propagated sd value using three biological replicates.