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. 2018 Sep 7;178(3):1310–1331. doi: 10.1104/pp.18.00603

Figure 2.

Figure 2.

Effects of mutations Z1 to Z4 on the functionality of full-length Rx1, either as the separate groups of mutations or in the combinations of Z1 with Z4 (Rx1 Z14) and Z2 with Z3 (Rx1 Z23). A, Rx1 constructs were coexpressed with the CP of PVX to assess their ability to induce elicitor-dependent cell death. The constructs were coexpressed with GFP to detect autoactivity. Images of the cell death response at 5 dpi are shown. The level of cell death was quantified by measuring the absorption of light at 655 nm by chlorophyll in a leaf extract (see “Materials and Methods”). A stronger cell death leads to lower chlorophyll levels. The error bars represent se (n = 8). Different letters represent significant differences (one-way ANOVA with a posthoc Tukey’s test, P < 0.05). B, Rx1-mediated resistance was tested by coexpressing the Rx1 constructs with a PVX:GFP amplicon and subsequent detection of the CP of PVX with an alkaline phosphatase-conjugated antibody in an ELISA at 5 dpi. The error bars represent se (n = 8). Statistical analysis is as in A.