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. 2018 Oct 8;178(3):1096–1111. doi: 10.1104/pp.18.00922

Figure 2.

Figure 2.

A, Functional characterization of the gene candidates PdCYP79D16, PdCYP71AN24, PdUGT85A19, PdUGT94AF1, PdUGT94AF2, and PdUGT94AF3 by transient expression in N. benthamiana and monitoring of product formation by LC-MS analysis and selected ion monitoring. When PdCYP79D16 and PdCYP71AN24 are coexpressed, small amounts of prunasin and prunasin-malonate ester can be detected, as compared with the control (p19). Amygdalin is detected only when at least three genes are coexpressed. B, Representative LC-MS recordings of the experiments shown on the gray background in A. Data are presented as means of three independent agroinfiltrated leaves ± sd.