Figure 5.

Phylogenetic neighbor-joining tree of 50 UGTs known to catalyze glycosylation of the Glc moiety in different classes of monoglucosides from different plant species. Based on the UGT gene data set, the derived UGT protein sequences were used to implement a multiple sequence alignment using the program ClustalV in MEGA7. The output files were used to construct the evolutionary history using the neighbor-joining method with 1,000 replications, where bootstrap values at the nodes are listed in percentage of the replications. UGT families known to harbor members catalyzing β1→2 or β1→6 bond formation are boxed in light blue. The UGT85 family catalyzing mandelonitrile glycosylation UGTs is shown in gray. The tree is a representation of the database utilized in the initial UGT search. A complete table including accession numbers is found in Supplemental Table S6.