FIG 4.
Flow cytometric analysis of phagocytosis and phagosome maturation in PBMC-DMs after infection with C. parapsilosis CLIB 214, CASEVO, ANDEVO, or MICAEVO strain. (A) Phagocytosing PBMC-DMs normalized to the phagocytic activity were measured and compared to the parental CLIB 214 strain. (B) Ratio of cells with phagolysosome colocalization, normalized to the proportion of phagosome maturation after the uptake of CLIB 214 cells. For each experiment, PBMC-DMs were utilized from five healthy human donors. A total of 2 × 104 PBMC-DMs were analyzed per donor for each strain. Significant differences were determined using Wilcoxon signed rank tests.