Fig. 4.

Overexpression of SKA3 facilitates the G1-S cell cycle transition and regulates the PI3K–Akt pathway in CC. a RNA-sequencing (RNA-Seq) analysis of genes in HeLa cells with stable SKA3 overexpression indicated involvement of the cell cycle. b KEGG pathway enrichment analysis (representative pathways) of genes in HeLa cells with stable SKA3 overexpression, including the PI3K–Akt pathway and cell cycle-associated genes. c Gene set enrichment analysis (GSEA) of HeLa cells with stable SKA3 overexpression for gene signatures of cell cycle- and d PI3K–Akt pathway-regulated genes. e Heat map of expression in the cell cycle gene set. f KEGG analysis of genes involved in the PI3K–Akt pathway and cell cycle