Table 1.
Primer sequences for the RT-PCR
| Gene | Primers | Sequence (5’-3’) | Size (bp) |
|---|---|---|---|
| β-actin | Sense | GGCTGTATTCCCCTCCATCG | 154 |
| Antisense | CCAGTTGGTAACAATGCCATGT | ||
| Solute carrier family 25 (carnitine/acylcarnitine translocase) member 20 (SLC25A20) | Sense | GGGGTCACTCCCATGTTTG | 135 |
| Antisense | TGTGGTGAATACGCCAGATAAC | ||
| Pyruvate dehydrogenase kinase isoform 4 (PDK4) | Sense | GGAGCATTTCTCGCGCTACA | 117 |
| Antisense | ACAGGCAATTCTTGTCGCAAA | ||
| Peroxisome proliferators activated receptors α (PPARα) | Sense | ATGGTGGACACGGAAAGCC | 124 |
| Antisense | CGATGGATTGCGAAATCTCTTGG | ||
| Carnitine palmitoyltransferase 1B (CPT1B) | Sense | GCGCCCCTTGTTGGATGAT | 112 |
| Antisense | CCACCATGACTTGAGCACCAG | ||
| acetyl-coenzyme A dehydrogenase 2 (ACAA2) | Sense | CTGCTCCGAGGTGTGTTTGTA | 105 |
| Antisense | GGCAGCAAATTCAGACAAGTCA | ||
| Acyl-coenzyme A dehydrogenase, long chain (ACADVL) | Sense | ACAGATCAGGTGTTCCCATACC | 114 |
| Antisense | CTTGGCGGGATCGTTCACTT | ||
| Carnitine palmitoyltransferase 1A (CPT1A) | Sense | TCCAGTTGGCTTATCGTGGTG | 98 |
| Antisense | TCCAGAGTCCGATTGATTTTTGC |