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. 2018 Nov 14;18:283. doi: 10.1186/s12870-018-1499-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Representative 2D proteome maps of soybean root nodules of control, Pb-treated (30 ppm) and Hg-treated (0.5 ppm). 400 μg of total protein was loaded on to 11 cm/non-linear/pH 4–7 IPG strips for first dimensional run on IEF cell followed by second dimensional run on 12% SDS-PAGE. The proteins were visualised by staining of gels with blue silver stain. The encircled protein spots with numbering represents differentially expressed proteins which were considered for tryptic digestion and subsequent identification through MALDI-TOF MS/MS