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Effect of urine matrix and duration of urine collection on the levels of DNA adducts in RT4 cells. (A and B) Urine from a single subject or PBS (40 mL) were spiked with 1 million RT4 cells pretreated with 4-ABP or AA-I at variable concentrations. (C) Urine from three subjects were incubated with 4-ABP (10 µM) or AA-I (0.1 µM) over T0, 10 or 24 h. N = 3 or 4 independent analyses for each subject. All measurements were reported as the mean ± SD.
