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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Eur J Neurosci. 2018 Sep 24;48(10):3255–3272. doi: 10.1111/ejn.14111

FIGURE 5.

FIGURE 5

Histological validation of viral vector expression in ilPFC and RE AAV vector constructs containing the inhibitory DREADD hM4Di (rAAV2-hSyn-HA-hM4Di-IRES-mCitrine) or EGFP only controls (rAAV2-hSyn-EGFP) were infused into ilPFC. Blue = DAPI, Green = EGFP, Red = anti- HA antibody throughout, as in A. (a) Representative coronal section demonstrating expression restricted to ilPFC. (b) Higher magnification image of section picture in A. Scale bar = 300 μm. (c) Superimposed traces of the maximal extent of EGFP expression across EGFP- only animals (green, top) anti- HA immunoreactivity across hM4Di animals (red, bottom) from all animals recorded. (d) Low-m agnification image demonstrating terminal expression throughout the midline thalamus in a EGFP animal with recording electrode tracks present in RE (white arrows). Scale bar = 900 μm. (e) Representative image of HA+ terminal labelling in RE (white arrows). Scale bar = 150 μm