Table 1. Characteristics of microsatellite loci used in this study.
Locus name, forward and reverse primer sequences, microsatellite genotypes (fragment length), fluorescent dye for U19, and GenBank accession number for each locus in Pocillopora acuta (Type 5 in mtORF) (see Nakajima et al., 2017). One colony consistently displayed two genotypes in the fragment analysis.
| Locus | Forward primer sequence (5′−3′) | Reverse primer sequence (5′−3′) | Microsatellite genotypes | Fluorescent dye for U19 | Accession No. | |
|---|---|---|---|---|---|---|
| 20 colonies | 4 colonies | |||||
| Psp_02 | CTGTGCTGGAATTCCCCTTA | U19-AGCCTACGGCGCAATAGTAG | 261/281 | 234/234 | FAM | LC222432 |
| Psp_16 | CCCGCTGCTGAGTAAGAATC | U19-AGAGAAACTGCAAAACCGC | 181/181 | 187/188 | FAM | LC222434 |
| Psp_18 | CACACGTTTTATGACAACGGA | U19-ATAAGCCGTAGGCCCTGTCT | 307/327 | 275/303 | FAM | LC222435 |
| Psp_23 | ACCATTGCCATCACTGTTCA | U19-TTCATTCATTCGTATTGGCG | 158/158 | 157/165 | VIC | LC222436 |
| Psp_29 | TTTCGTACCAAAATCCAGGC | U19-TTTTTCAGTCGCAAGAGGC | 257/262 | 257/257 | VIC | LC222437 |
| Psp_32 | AAGCACGCAATTCAGCCTAT | U19-AGCCTAAGACGAATCGAGCA | 127/147 | 127/127 | VIC | LC222438 |
| Psp_33 | CCATTTCCCGAATCTCTCTC | U19-CTCGTCGCCCAGATATAAA | 258/266 | 256/270 | NED | LC222439 |
| Psp_39 | TCTTTACAGCACAGGAGCCA | U19-TTTTTCTTGCGGTCCAATTC | 121/121 | 121/121 | NED | LC222441 |
| Psp_48 | TGTAAATTCAAGAGAATGGGCA | U19-GTTTCCTGATGGTGTTCT | 183/183 | 183/191 | NED | LC222443 |