Figure 4.

Left: Schematic representation of the DNA hairpin array design containing a single RNA insert (in italic) and the chemical outcome of enzymatic cleavage mediated by RNase HII. In red is the 5‐nt long variable region. The cleavage site is represented by an arrow. M stands for the complementary nucleotide to the dN or rN nucleotides. Hairpin sequences below represent the best and worst substrates for RNase HII‐mediated cleavage. Right: Sequence motifs assembled from the collection of the top 20 most‐ and least‐cleaved hairpin sequences.